Quantitative Analysis of Chemicals Using Spectrophotometer

Reviewed by Anurag Mishra (Sr. Technical Consultant)

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Chemical mixtures absorb, transmits and reflect light in a particular range of wavelength. Spectrophotometer measures how much a chemical substance absorbs and transmits. It is widely used in quantitative analysis in several fields such as biochemistry, industrial applications, physics, chemistry etc.


  • In biochemistry, spectrophotometers are used to determine enzyme-catalysed reactions.
  • In clinical application, it can be used to test blood or tissues for clinical diagnosis.
  • Used in agriculture and soil quality analysis. It includes a qualitative analysis of phosphates and nitrogen in fertilizer products.
  • Proteins tend to absorb light at a particular wavelength. A spectrophotometer measures the concentration of a purified protein in solution.

Portable Spectrophotometer measures the amount of intensity of light absorbed after it passes through the sample solution. Using this instrument, the amount of chemical substance can be detected by evaluating the intensity of light.

Types of a spectrophotometer are:

  • UV-visible spectrophotometer: It uses light in the UV range (185 -400 nm) and visible range (400-700nm) of the electromagnetic radiation spectrum. It can be used to study biological macromolecules.
  • IR spectrophotometer: It uses light in the infrared range (700-15000 nm) of the electromagnetic radiation spectrum. It can be used to study chemicals.

In visible spectrophotometry, the absorption or the transmission of a particular substance is determined by observed color. For example, a solution sample that absorbs light over all visible ranges will appear black. If all wavelengths are transmitted which means it absorbs nothing, the solution sample will appear white. If a solution sample absorbs red light near to 700 nm, it will appear green as green is the complementary color of red.  Visible spectrophotometers, use a prism to reduce a range of wavelength so that the given beam of light is passed through a solution sample.

TP 800 Spectrophotometer is an instrument that measures an amount of light that a sample can absorb. The working principle is very simple. A light beam passes through a sample that measures the light intensity of a sample. It is used to measure color and monitoring color accuracy all through the process of production. These equipment are used by researchers and manufacturers all over the world for applications in industries and commercial purposes.  It is used to measure color absorption in metals, fabrics, plastics etc. It helps in assuring that the color selected remains consistent from its actual conception to the finished product.

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